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Can't access MySite pages all of a sudden. Get 403 Forbidden error

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It was working great for over a month but today I came in and I cannot access any of the MySites pages.  They are on their own web application on port 8080.  Last thing I did was make a copy of the the master pages and export them for editing, but I don't recall touching the originals at all so I'm not too sure if that's related.

I used IIS Manager to check the file permissions and they are exactly the same as my other SP Web Applications, so I'm guessing that is not the issue.  I have tried IIS Reset and recycling the application pool but neither helped.

Here is what I'm seeing in the ULS:

A runtime exception was detected. Details follow.  Message: Access to the path 'C:\inetpub\wwwroot\wss\VirtualDirectories\8080\bin' is denied.  Technical Details: System.UnauthorizedAccessException: Access to the path 'C:\inetpub\wwwroot\wss\VirtualDirectories\8080\bin' is denied.     at System.IO.__Error.WinIOError(Int32 errorCode, String maybeFullPath)     at System.IO.FileSystemEnumerableIterator`1.CommonInit()     at System.IO.FileSystemEnumerableIterator`1..ctor(String path, String originalUserPath, String searchPattern, SearchOption searchOption, SearchResultHandler`1 resultHandler, Boolean checkHost)     at System.IO.DirectoryInfo.InternalGetFiles(String searchPattern, SearchOption searchOption)     at System.Web.Configuration.CompilationSection.LoadAllAssembliesFromAppDomainBinDirectory()    at System.Web.Configuration.AssemblyInfo.get_AssemblyInternal()     at System.Web.Compilation.BuildManager.GetReferencedAssemblies(CompilationSection compConfig)     at System.Web.UI.ScriptManager.get_DefaultAjaxFrameworkAssembly()    at System.Web.UI.ScriptManager..ctor()     at ASP.MYSITE15_MASTER__373277105.__BuildControlScriptManager()     at ASP.MYSITE15_MASTER__373277105.__BuildControl__control29()     at ASP.MYSITE15_MASTER__373277105.__BuildControlSPHtmlTag()    at ASP.MYSITE15_MASTER__373277105.__BuildControlTree(MYSITE15_MASTER__373277105 __ctrl)     at System.Web.UI.MasterPage.CreateMaster(TemplateControl owner, HttpContext context, VirtualPath masterPageFile, IDictionary contentTemplateCollection)    at System.Web.UI.Page.ApplyMasterPage()     at System.Web.UI.Page.PerformPreInit()     at System.Web.UI.Page.ProcessRequestMain(Boolean includeStagesBeforeAsyncPoint, Boolean includeStagesAfterAsyncPoint)

Not too familiar with IIS and I can't seem to figure this one out.  Any help would be appreciated!


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